Reporter

Part:BBa_K325100:Design

Designed by: Ben Reeve and Paul Masset   Group: iGEM10_Cambridge   (2010-09-15)


EPIC Firefly Luciferase and LRE
P. Pyralis
(E. coli optimised)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2580
    Illegal BamHI site found at 948
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2284


Design Notes

It was ensured that no unwanted restriction sites were created during optimisation. A BamH1 site was added between the two genes.


Source

Codon optimised and synthesised by DNA 2.0

References